A pre-in vitro maturation medium containing cumulus oocyte complex ligand-receptor signaling molecules maintains meiotic arrest, supports the cumulus oocyte complex and improves oocyte developmental competence
نویسندگان
چکیده
منابع مشابه
The extracellular calcium-sensing receptor is expressed in the cumulus-oocyte complex in mammals and modulates oocyte meiotic maturation.
The extracellular calcium-sensing receptor (CASR) plays an important role in cells involved in calcium (Ca2+) homeostasis by directly sensing changes in the extracellular Ca2+ ion concentration. We previously reported the localization and quantitative expression of CASR protein in human oocytes. In this study, we examined the expression and the functional role of CASR during oocyte meiotic matu...
متن کاملAltered composition of the cumulus-oocyte complex matrix during in vitro maturation of oocytes.
BACKGROUND In vitro maturation (IVM) of mammalian oocytes has potential health benefits for patients undergoing assisted reproduction as an alternative to gonadotrophin treatment. This procedure is also useful for studying the process of oocyte and early embryo development. However, oocytes undergoing IVM have much lower competence than in vivo matured oocytes. Efforts to optimize IVM success h...
متن کاملEffect of Cumulus Oocyte Complex Morphology on in Vitro Maturation of Bovine Oocytes*
An experimental design was framed to analyse the effect of cumulus oocyte complex (COC) morphology on in vitro maturation of bovine oocytes. Slaughter house derived bovine ovaries from South Indian breeds and crossbred cattle of Kerala were subjected to three retrieval methods to yield different quality grades of oocytes. Based on the number of layers of cumulus cells and ooplasm character, the...
متن کاملThe human cumulus--oocyte complex gene-expression profile.
BACKGROUND The understanding of the mechanisms regulating human oocyte maturation is still rudimentary. We have identified transcripts differentially expressed between immature and mature oocytes and cumulus cells. METHODS Using oligonucleotide microarrays, genome-wide gene expression was studied in pooled immature and mature oocytes or cumulus cells from patients who underwent IVF. RESULTS...
متن کاملExpression of interferon regulatory factor-1 in the mouse cumulus-oocyte complex is negatively related with oocyte maturation
OBJECTIVE We found previously that interferon regulatory factor (Irf)-1 is a germinal vesicle (GV)-selective gene that highly expressed in GV as compared to metaphase II oocytes. To our knowledge, the function of Irf-1 in oocytes has yet to be examined. The present study was conducted to determine the relationship between retinoic acid (RA) and RA-mediated expression of Irf-1 and the mouse oocy...
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ژورنال
عنوان ژورنال: MHR: Basic science of reproductive medicine
سال: 2017
ISSN: 1360-9947,1460-2407
DOI: 10.1093/molehr/gax032